A comparative study of the antifungal property of tanglad (cymbopogon citratus, poaceae) and kuchai (alliumodorum, liliaceae) extract formulated as cream and oral gel / Ara Mae F. Barona, Blesserie A. Corpuz, Vibelle M. Peleno, Gennifer B. Ragpala; faculty researcher, Ghinelli Anne P. Laygo

Thesis (unpublished)--Lorma Colleges, San Fernando City, La Union, 2018.

Includes bibliographical references.

Fungal infections are one of the major problems associated with our daily life. But unfortunately, despite having knowledge that it could be life threatening they are still one of the most neglected diseases. This research study aimed to the evaluation of the antifungal activity of the leaf extract against Trichophyton rubrum and Candida albicans, the comparison of the antifungal activity of the leaf extracts to that of the reference standard or positive control drugs which is commercially available antifungal cream and oral gel, and also this study aimed to evaluate the level of safety of the formulated plain Tanglad and combined Tanglad & Kuchai Cream and Oral Gel in comparison with the previous study of Lubuguin D., et.al. (2014), The Antifungal Cream from the Crude Drug Extracts of Kuchai (Allium odorum, Liliacea) Leaves.
The study utilized the experimental method in comparing the antifungal property of various plant extracts from the positive control drug and to prove the susceptibility of the test organism with the prepared dilutions of the extracts. Tanglad (Cymbopogan citratus, Poacease) leaves and KUchai ( Allium odorum, Liliaceae) leaves extract were subjected to phytochemical screening, which includes, MIC test, MFC test and Well Diffusion Method to determine the antifungal activity of Cymbopogon citratus and Allium odorum against Trichophyton rubrum and Candida albicans. The formulated creams and oral gels of various crude extracts were used in the evaluation of dermal sensitivity through Patch and Scratch using acclimatized guinea pigs for evaluation of its safety.

Results and Discussions. The data and results that were gathered during experiments were statistically compared between the formulated creams and oral gels of 100% combined Tanglad and Kuchai leaf extracts exhibited the most satisfying result of all. The results reveal that the formulated creams and oral gels of the various plant extracts were significant to inhibit fungal growth and absence of skin toxicity. Through formulation of this cream, potential adverse effect can be avoided.

Conclusions. The crude leaves extract dilution of combined Tanglad and Kuchai shows the most effective concentration that could inhibit fungal growth, among all the antifungal screening- MIC test, MFC test, and Well Diffusion Methods against Trichophyton rubrum and Candida albicans. The formulated creams and oral gels of plain Tanglad, and combined Tanglad & Kuchai were also proven safe for topical administration based on the results of the Patch and Scratch test.